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Enzymatic O-methylation of catechol estrogens in red blood cells: differences in animal species and strains.
- Source :
-
Steroids [Steroids] 1993 Nov; Vol. 58 (11), pp. 540-6. - Publication Year :
- 1993
-
Abstract
- Enzymatic O-methylation of catechol estrogens in red blood cells has been investigated with respect to species difference. In the presence of S-adenosylmethionine, 2- or 4-hydroxyestradiol (2-OHE2 or 4-OHE2) was incubated with blood lysate obtained from rats (five strains), guinea pigs, mice, rabbits, dogs, monkeys, and humans, respectively. The yielded guaiacols and unchanged substrate were determined by gas chromatography/mass spectrometry in a selected ion monitoring mode employing the corresponding 2H4-labeled compounds as internal standards. The total amounts of guaiacols formed from 2-OHE2 and 4-OHE2 were different, being the highest (79.6% and 38.1%) in monkeys and the lowest (5.1% and 1.9%) in humans. The ratios of isomeric guaiacols formed from 4-OHE2 (4Me/3Me) were 7.6-71, while those from 2-OHE2 (2Me/3Me) were 1.4-3.2. Thus, marked differences in O-methylation of catechol estrogens were observed among animal species, but no significant strain difference was detected in rats.
- Subjects :
- Adult
Animals
Dogs
Estradiol analogs & derivatives
Estradiol metabolism
Guinea Pigs
Humans
Macaca fascicularis
Methylation
Mice
Mice, Inbred ICR
Rats
Rats, Inbred F344
Rats, Inbred SHR
Rats, Sprague-Dawley
Rats, Wistar
S-Adenosylmethionine metabolism
Species Specificity
Erythrocytes enzymology
Estrogens, Catechol blood
Subjects
Details
- Language :
- English
- ISSN :
- 0039-128X
- Volume :
- 58
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Steroids
- Publication Type :
- Academic Journal
- Accession number :
- 8273118
- Full Text :
- https://doi.org/10.1016/0039-128x(93)90032-i