Back to Search
Start Over
CD and FTIR studies of an immunogenic disulphide cyclized octadecapeptide, a fragment of a snake curaremimetic toxin.
- Source :
-
Biochimica et biophysica acta [Biochim Biophys Acta] 1993 Dec 08; Vol. 1203 (2), pp. 191-8. - Publication Year :
- 1993
-
Abstract
- In a previous paper, the systematic epitope screening of a snake curaremimetic toxin or toxin a was described by this group using a panel of synthetic octadecapeptides. The disulphide cyclized peptide (Cys-23,40)(23-40) corresponding to loop II of the native toxin was found to elicit, with no linkage to a carrier, neutralizing antisera against the toxin. We have now undertaken the conformational study of this immunogenic disulphide cyclized peptide by CD and FTIR. The CD study of the peptide was carried in aqueous solution under various conditions (pH, temperature, presence of micelles) and in trifluoroethanol solution. Low temperature, SDS micelles and trifluoroethanol were found to induce a beta-sheet formation (16 to 39%). FTIR spectra of the peptide in the solid state (dry film) and in D2O solution or deuterated-TFE solution (hydrated film) displayed some characteristic bands indicating the presence of beta-sheet (1623 cm-1) and beta-turn (1637 cm-1; 1694 cm-1). These studies indicate that the immunogenic disulphide cyclized peptide (23-40) can adopt in solution an ordered structure.
- Subjects :
- Amino Acid Sequence
Animals
Circular Dichroism
Cobra Neurotoxin Proteins immunology
Curare immunology
Fourier Analysis
Molecular Sequence Data
Neutralization Tests
Protein Conformation
Solutions
Temperature
Cobra Neurotoxin Proteins chemistry
Curare chemistry
Disulfides chemistry
Peptide Fragments chemistry
Peptides, Cyclic chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 1203
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 8268199
- Full Text :
- https://doi.org/10.1016/0167-4838(93)90082-3