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Comparison of clearance and metabolism of infused cholecystokinins 8 and 58 in dogs.
- Source :
-
Gastroenterology [Gastroenterology] 1993 Dec; Vol. 105 (6), pp. 1732-6. - Publication Year :
- 1993
-
Abstract
- Background: Cholecystokinin (CCK) 58 is the predominant molecular form of CCK in canine and human intestine and circulating blood. There is no report on the metabolism and clearance rate of CCK-58. The aim of this study was to compare the in vivo half-life and metabolism of CCK-58 with that of synthetic CCK-8.<br />Methods: CCK-58 was purified from canine intestine by consecutive high-performance liquid chromatographic (HPLC) and fast protein liquid chromatographic steps. The peptides were given to 12 dogs as an intravenous (IV) bolus injection to determine the half-life of circulating CCK. Six dogs were given CCK-58 or CCK-8 as a constant IV infusion to determine plasma clearance rates and stability in circulating blood. Circulating molecular forms of CCK were determined by radioimmunoassay after extraction of CCK from plasma and characterization by HPLC.<br />Results: The half-life of CCK-58 was 4.4 +/- 0.6 minutes compared with 1.3 +/- 0.1 minutes for CCK-8. Less than 5% of CCK-58 could be detected as smaller forms during constant IV infusion.<br />Conclusions: The longer half-life of CCK-58 compared with CCK-8 and the minimal conversion into smaller forms during constant IV infusion are consistent with the finding that CCK-58 is not only the major stored form but also the circulating form of CCK after endogenous stimulation in dogs.
- Subjects :
- Animals
Cholecystokinin administration & dosage
Cholecystokinin isolation & purification
Dogs
Half-Life
Infusions, Intravenous
Injections, Intravenous
Intestinal Mucosa chemistry
Metabolic Clearance Rate
Sincalide administration & dosage
Sincalide chemical synthesis
Cholecystokinin pharmacokinetics
Sincalide pharmacokinetics
Subjects
Details
- Language :
- English
- ISSN :
- 0016-5085
- Volume :
- 105
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 8253350
- Full Text :
- https://doi.org/10.1016/0016-5085(93)91070-x