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Activation of a calcium magnesium independent endonuclease in human leukemic cell apoptosis.
- Source :
-
Anticancer research [Anticancer Res] 1993 Sep-Oct; Vol. 13 (5A), pp. 1253-9. - Publication Year :
- 1993
-
Abstract
- Cytotoxic drugs induce apoptosis in human tumour cell lines and this is characterised by fragmentation of the cell's DNA into nucleosome size units or multiples thereof. In the present study we demonstrated that nuclei isolated from three human haematopoietic cell lines, HL-60, U937 and K562, contain an endonuclease that is independent of Ca++, Mg++ and Na+ ions for its activity. This contrasts with what has previously been shown for a number of rodent cell types in which apoptosis has been studied. The lack of ion sensitivity is also found in the nuclei of peripheral blood granulocytes, indicating the data are not peculiar to cell lines. In addition, this particular endonuclease activity does not appear to be sensitive to the endonuclease inhibitor aurintricarboxylic acid. The previously demonstrated lack of calcium flux in HL-60 cells undergoing apoptosis, and the current demonstration of a lack of an endonuclease dependent on this ion for its activity, suggest that the mechanism of apoptosis in human cells may be different from that in rodent cells.
- Subjects :
- Calcium pharmacology
Camptothecin pharmacology
DNA, Neoplasm analysis
DNA, Neoplasm drug effects
Dactinomycin pharmacology
Electrophoresis, Agar Gel
Endonucleases drug effects
Endonucleases isolation & purification
Enzyme Activation
Etoposide pharmacology
Granulocytes enzymology
Humans
Leukemia, Monocytic, Acute pathology
Leukemia, Myelogenous, Chronic, BCR-ABL Positive pathology
Leukemia, Promyelocytic, Acute pathology
Magnesium pharmacology
Tumor Cells, Cultured
Zinc pharmacology
Apoptosis physiology
Endonucleases biosynthesis
Leukemia, Monocytic, Acute enzymology
Leukemia, Myelogenous, Chronic, BCR-ABL Positive enzymology
Leukemia, Promyelocytic, Acute enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0250-7005
- Volume :
- 13
- Issue :
- 5A
- Database :
- MEDLINE
- Journal :
- Anticancer research
- Publication Type :
- Academic Journal
- Accession number :
- 8239494