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Cytotoxic activity of rev protein of human immunodeficiency virus type 1 by nucleolar dysfunction.
- Source :
-
Experimental cell research [Exp Cell Res] 1993 Nov; Vol. 209 (1), pp. 89-102. - Publication Year :
- 1993
-
Abstract
- The rev protein (Rev) of the human immunodeficiency virus type 1 (HIV-1) is known as a post-transcriptional regulator of viral gene expression. It is located in the cell nucleolus. Transiently expressed Rev caused nucleolar ballooning and deformity with aberrant accumulation of rRNAs, and de novo synthesis of rRNAs decreased dramatically in these cells. However, similarly expressed rex protein (Rex) of the human T-cell leukemia virus type I, which is a functional homologue to Rev, did not affect nucleolar structure and function. Rev expression resulted in cell death with nucleolar destruction in an inducible cell line. Analysis of Rev mutants revealed that both the nucleolar targeting signal of Rev and the multimerization domain are prerequisites to the nucleolar disintegration by Rev. Human T-cells acutely infected with HIV-1 contained nucleoli which were deformed and filled with Rev, but chronically infected cells had intact nucleoli. Involvement of Rev in cytopathic effects in HIV-1 infection is discussed.
- Subjects :
- Base Sequence
Blotting, Western
Cell Death
Cell Line
Cell Nucleolus ultrastructure
Fluorescent Antibody Technique
Gene Products, rex metabolism
Humans
Molecular Sequence Data
Mutation
RNA, Ribosomal metabolism
T-Lymphocytes microbiology
rev Gene Products, Human Immunodeficiency Virus
Cell Nucleolus drug effects
Cytopathogenic Effect, Viral
Gene Products, rev pharmacology
HIV-1 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0014-4827
- Volume :
- 209
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Experimental cell research
- Publication Type :
- Academic Journal
- Accession number :
- 8224012
- Full Text :
- https://doi.org/10.1006/excr.1993.1289