Back to Search
Start Over
Clarification of subtle reciprocal rearrangements using fluorescence in situ hybridization.
- Source :
-
American journal of medical genetics [Am J Med Genet] 1993 Aug 15; Vol. 47 (2), pp. 223-30. - Publication Year :
- 1993
-
Abstract
- Fluorescence in situ hybridization (FISH) using chromosome-specific DNA libraries as painting probes was applied in the analysis of six subtle, balanced chromosome rearrangements. Both fresh and older slides, some of which had been previously G-banded, were used to determine if FISH could identify unambiguously very small amounts of translocated material. Our results indicate that this procedure can clearly and precisely distinguish the specific components of extremely subtle translocations, in different cell types, such as leukocytes, aminocytes, and chorionic villus, and irregardless of preparation age. This ability makes FISH a valuable tool in clinical cytogenetics for the confirmation of preliminary G-banded karyotypes.
- Subjects :
- Chromosomes, Human, Pair 1
Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 18
Chromosomes, Human, Pair 22
Chromosomes, Human, Pair 4
Chromosomes, Human, Pair 7
Chromosomes, Human, Pair 8
Chromosomes, Human, Pair 9
DNA Probes
Female
Gene Library
Humans
Male
Pregnancy
Sensitivity and Specificity
In Situ Hybridization methods
Prenatal Diagnosis methods
Translocation, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 0148-7299
- Volume :
- 47
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- American journal of medical genetics
- Publication Type :
- Academic Journal
- Accession number :
- 8213910
- Full Text :
- https://doi.org/10.1002/ajmg.1320470217