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Perturbation of cell adhesion and microvilli formation by antisense oligonucleotides to ERM family members.
- Source :
-
The Journal of cell biology [J Cell Biol] 1994 Jun; Vol. 125 (6), pp. 1371-84. - Publication Year :
- 1994
-
Abstract
- To examine the functions of ERM family members (ezrin, radixin, and moesin), mouse epithelial cells (MTD-1A cells) and thymoma cells (L5178Y), which coexpress all of them, were cultured in the presence of antisense phosphorothioate oligonucleotides (PONs) complementary to ERM sequences. Immunoblotting revealed that the antisense PONs selectively suppressed the expression of each member. Immunofluorescence microscopy of these ezrin, radixin, or moesin "single-suppressed" MTD-1A cells revealed that the ERM family members are colocalized at cell-cell adhesion sites, microvilli, and cleavage furrows, where actin filaments are densely associated with plasma membranes. The ezrin/radixin/moesin antisense PONs mixture induced the destruction of both cell-cell and cell-substrate adhesion, as well as the disappearance of microvilli. Ezrin or radixin antisense PONs individually affected the initial step of the formation of both cell-cell and cell-substrate adhesion, but did not affect the microvilli structures. In sharp contrast, moesin antisense PONs did not singly affect cell-cell and cell-substrate adhesion, whereas it partly affected the microvilli structures. These data indicate that ezrin and radixin can be functionally substituted, that moesin has some synergetic functional interaction with ezrin and radixin, and that these ERM family members are involved in cell-cell and cell-substrate adhesion, as well as microvilli formation.
- Subjects :
- Animals
Base Sequence
Blood Proteins genetics
Cell Adhesion drug effects
Fluorescent Antibody Technique
Leukemia L5178
Membrane Proteins genetics
Mice
Microscopy, Electron, Scanning
Microvilli drug effects
Microvilli ultrastructure
Molecular Sequence Data
Phosphoproteins genetics
Proteins genetics
Thionucleotides pharmacology
Thymoma ultrastructure
Cell Adhesion physiology
Cytoskeletal Proteins
DNA-Binding Proteins genetics
Microfilament Proteins
Microvilli physiology
Oligonucleotides, Antisense pharmacology
Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9525
- Volume :
- 125
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 8207064
- Full Text :
- https://doi.org/10.1083/jcb.125.6.1371