Oxidation of pyridine nucleotides and depletion of ATP and ADP during calcium- and inorganic phosphate-induced mitochondrial permeability transition.

We have examined the pyridine and adenine nucleotide status during calcium- and inorganic phosphate-induced permeability transition. Calcium- and inorganic phosphate-induced permeability transition is accompanied by the rapid oxidation of pyridine nucleotides and depletion of ATP and ADP with conversion to AMP. The addition of cyclosporin A, a potent inhibitor of the permeability transition prevented the oxidation of pyridine nucleotides and depletion of ATP and ADP.