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The rat vasoactive intestinal polypeptide cyclic AMP response element regulates gene transcriptional responses differently in neonatal and adult rat sensory neurons.
- Source :
-
Neuroscience letters [Neurosci Lett] 1994 Feb 14; Vol. 167 (1-2), pp. 19-23. - Publication Year :
- 1994
-
Abstract
- We studied the ability of the rat vasoactive intestinal polypeptide (VIP) cyclic AMP responsive element (CRE) to regulate reporter gene expression through a c-fos promoter in rat sensory neurons transfected in culture by plasmid microinjection. The CRE enhanced the synergistic response of the promoter to combined potassium-evoked depolarisation and forskolin treatment in neonatal but not adult rat neurons. This corresponds to endogenous VIP expression which is induced synergistically by the same stimuli in neonatal but not adult rat neurons. We conclude that VIP expression in sensory neurons, which is induced by axotomy in vivo, could be regulated through the CRE.
- Subjects :
- Animals
Base Sequence
Chloramphenicol O-Acetyltransferase genetics
Chloramphenicol O-Acetyltransferase metabolism
Colforsin pharmacology
Electrophysiology
Genes, Reporter
Molecular Sequence Data
Oligonucleotide Probes genetics
Potassium pharmacology
Rats
Aging physiology
Animals, Newborn physiology
Cyclic AMP Response Element-Binding Protein physiology
Neurons, Afferent physiology
Transcription, Genetic physiology
Vasoactive Intestinal Peptide pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0304-3940
- Volume :
- 167
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Neuroscience letters
- Publication Type :
- Academic Journal
- Accession number :
- 8177522
- Full Text :
- https://doi.org/10.1016/0304-3940(94)91018-9