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Identification of a common clonal human immunodeficiency virus integration site in human immunodeficiency virus-associated lymphomas.
- Source :
-
Cancer research [Cancer Res] 1994 Apr 15; Vol. 54 (8), pp. 2069-72. - Publication Year :
- 1994
-
Abstract
- Infection with human immunodeficiency virus type 1 (HIV-1) is associated with a high incidence of lymphoma. Typically, the lymphomas are B-cell in origin, and although they occur in the setting of HIV-1 infection, historical studies have found no evidence for the presence of HIV-1 within the transformed B-cells. We describe a new class of large cell lymphoma wherein HIV p24 expression within the tumor specimens was found to be extremely high. In the first case, HIV was expressed in the tumor-associated transformed T-cells. In three other cases, HIV was found to be highly expressed in tumor-associated macrophages. These tumors exhibited a mixed immunophenotype histologically. Analysis by inverse polymerase chain reaction, using HIV long terminal repeat primers, demonstrated monoclonal HIV integration sites for all four tumors. Direct sequencing of the T-cell lymphoma inverse polymerase chain reaction products identified the HIV integration site within the fur gene, just upstream from the c-fes/fps protooncogene. Using segments of the fur gene as a probe, the other three monoclonal integration sites mapped to the same region. Although the integration and up-regulation of c-fes/fps was localized to the tumor cells within the T-cell lymphoma, the cells containing the monoclonal HIV in the other mixed immunophenotype lymphomas are currently unknown. These observations suggest that HIV may contribute directly to lymphomagenesis and identify a common site of HIV integration within a subset of acquired immunodeficiency syndrome lymphoma.
- Subjects :
- Base Sequence
DNA Primers
Humans
Immunophenotyping
Lymphoma, AIDS-Related immunology
Lymphoma, AIDS-Related pathology
Lymphoma, B-Cell genetics
Lymphoma, B-Cell microbiology
Lymphoma, B-Cell pathology
Molecular Sequence Data
Oligonucleotide Probes
Polymerase Chain Reaction
Protein-Tyrosine Kinases genetics
Proto-Oncogene Proteins genetics
Proto-Oncogene Proteins c-fes
Restriction Mapping
HIV-1 genetics
Lymphoma, AIDS-Related genetics
Lymphoma, AIDS-Related microbiology
Proto-Oncogenes
Virus Integration
Subjects
Details
- Language :
- English
- ISSN :
- 0008-5472
- Volume :
- 54
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Cancer research
- Publication Type :
- Academic Journal
- Accession number :
- 8174106