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Sensitivity of the fibrinogen clotting time: an in vitro test of potential thrombogenicity.
- Source :
-
Vox sanguinis [Vox Sang] 1994; Vol. 66 (1), pp. 1-7. - Publication Year :
- 1994
-
Abstract
- The fibrinogen clotting time (FCT) is a measure of thrombin activity, and is used to evaluate the potential thrombogenicity of prothrombin complex concentrates (PCC). We have defined end points for clot formation in this test which allow the measurement in PCC of thrombin concentrations as low as 0.001 IU/ml. The FCT of thrombin and PCC samples which did not contain antithrombin III (ATIII) were the same when measured at 20 degrees C or 37 degrees C. In the presence of ATIII (0.05 or 0.25 IU/ml), samples of PCC which were known to contain thrombin showed shorter FCT at 20 degrees C than at 37 degrees C. Inclusion of both ATIII (0.25 IU/ml) and heparin (4 IU/ml) in PCC ensured the complete inactivation of endogenous thrombin.
- Subjects :
- Antithrombin III metabolism
Benzamidines pharmacology
Blood Coagulation drug effects
Blood Coagulation Factors adverse effects
Blood Coagulation Factors chemistry
Heparin pharmacology
Hirudins pharmacology
Humans
Phenylalanine analogs & derivatives
Phenylalanine pharmacology
Piperidines pharmacology
Reproducibility of Results
Temperature
Thrombin metabolism
Thrombosis etiology
Blood Coagulation Factors standards
Blood Coagulation Tests
Fibrinogen metabolism
Thrombin analysis
Thrombosis prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 0042-9007
- Volume :
- 66
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Vox sanguinis
- Publication Type :
- Academic Journal
- Accession number :
- 8146976
- Full Text :
- https://doi.org/10.1111/j.1423-0410.1994.tb00268.x