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Bcl-2 expression promotes B- but not T-lymphoid development in scid mice.
- Source :
-
Nature [Nature] 1994 Mar 31; Vol. 368 (6470), pp. 457-60. - Publication Year :
- 1994
-
Abstract
- Expression of antigen receptors is vital for the development of B and T lymphocytes. In mice with the scid mutation, which are unable to make productive rearrangements of their immunoglobulin and T-cell receptor (TCR) genes, lymphopoiesis aborts at an early stage. The death of the immature lymphocytes by apoptosis is postulated to result from a failure to receive a survival signal induced by receptor engagement. Consistent with this hypothesis, introduction of immunoglobulin or TCR transgenes into scid mice promoted an increase in B- or T-lymphoid cells, respectively. As the protein encoded by the bcl-2 gene can inhibit cell death, we tested whether lymphopoiesis could be rescued in scid mice by crossing in a bcl-2 transgene. Strikingly, the bcl-2/scid mice accumulated almost normal numbers of B-lymphoid cells which lacked surface immunoglobulin but expressed markers of maturity. T-cell development remained blocked. Introducing a TCR transgene enabled bcl-2/scid mice to develop normal numbers of CD4+8+ thymocytes even in the absence of immunological selection, suggesting that T cells become competent to respond to bcl-2 protein only after the TCR complex is displayed at the cell surface.
- Subjects :
- Animals
Antigens, Differentiation biosynthesis
Base Sequence
Bone Marrow Cells
Cell Differentiation
Cell Survival
Cells, Cultured
DNA Primers
Immunophenotyping
Mice
Mice, SCID
Mice, Transgenic
Molecular Sequence Data
Proto-Oncogene Proteins genetics
Proto-Oncogene Proteins c-bcl-2
Receptors, Antigen, B-Cell biosynthesis
Receptors, Antigen, T-Cell biosynthesis
Spleen cytology
B-Lymphocytes cytology
Proto-Oncogene Proteins biosynthesis
T-Lymphocytes cytology
Subjects
Details
- Language :
- English
- ISSN :
- 0028-0836
- Volume :
- 368
- Issue :
- 6470
- Database :
- MEDLINE
- Journal :
- Nature
- Publication Type :
- Academic Journal
- Accession number :
- 8133891
- Full Text :
- https://doi.org/10.1038/368457a0