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Embryonic Purkinje cells grafted on the surface of the adult uninjured rat cerebellum migrate in the host parenchyma and induce sprouting of intact climbing fibres.
- Source :
-
The European journal of neuroscience [Eur J Neurosci] 1994 Jan 01; Vol. 6 (1), pp. 121-36. - Publication Year :
- 1994
-
Abstract
- By grafting solid pieces of cerebellar anlage onto the surface of the adult rat cerebellum, we have investigated the problem of the interactions between embryonic and adult neurons in an intact brain. A few days after grafting, embryonic astrocytic processes crossed the graft--host interface and radiated into the recipient molecular layer. Several grafted Purkinje cells also migrated into the host brain along such processes as well as adult Bergmann glia. Adult climbing fibres, labelled by means of Phaseolus vulgaris leucoagglutinin (PHA-L), sprouted new collateral branches which terminated on embryonic Purkinje cells at both extra- and intraparenchymal levels. No sign of activation of host astroglia or microglia was evident in the host cerebellum in relation to these processes. Embryonic Purkinje cells which migrated into the host cerebellum developed an adult-like morphology. Intraparenchymal grafts of neocortical embryonic tissue induced conspicuous growth of host olivary axons, characterized by a pattern which was different from that observed following cerebellar grafts. By contrast, when neocortical tissue was placed onto the surface of the recipient cerebellum, graft--host interactions were limited and climbing fibre sprouting was rarely seen. These results show that (i) supernumerary Purkinje cells can penetrate and settle in the adult intact cerebellar cortex, (ii) adult climbing fibres are able to innervate these new targets in the absence of any injury or activation of non-neuronal cells of the adult brain, and (iii) in the absence of damage to the adult brain, the plasticity of adult olivary axons is specifically elicited and controlled by embryonic Purkinje cells.
- Subjects :
- Animals
Cerebellum cytology
Female
Graft vs Host Reaction physiology
Immunohistochemistry
Neuronal Plasticity physiology
Olivary Nucleus cytology
Olivary Nucleus physiology
Phytohemagglutinins
Pregnancy
Rats
Rats, Wistar
Brain Tissue Transplantation physiology
Cell Transplantation physiology
Cerebellum physiology
Fetal Tissue Transplantation physiology
Nerve Fibers physiology
Purkinje Cells physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0953-816X
- Volume :
- 6
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The European journal of neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 8130928
- Full Text :
- https://doi.org/10.1111/j.1460-9568.1994.tb00254.x