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Histaminergic regulation of antibody-dependent cellular cytotoxicity of granulocytes, monocytes, and natural killer cells.
- Source :
-
Journal of leukocyte biology [J Leukoc Biol] 1994 Mar; Vol. 55 (3), pp. 392-7. - Publication Year :
- 1994
-
Abstract
- We investigated effects of the biogenic diamine histamine on antibody-dependent cellular cytotoxicity (ADCC) against autologous anti-D-coated red blood cells mediated by human granulocytes, monocytes, and natural killer (NK) cells. Effector cells were separated from peripheral blood by countercurrent centrifugal elutriation. ADCC of monocytes and neutrophilic granulocytes was suppressed by histamine. ADCC of enriched CD3-/56+ NK cells was unchanged by histamine. ADCC of NK cells was effectively inhibited by elutriated monocytes or neutrophils. Histamine completely reversed the inhibition of NK cell-mediated ADCC induced by monocytes and partly reversed the inhibition induced by neutrophils; thereby, histamine augmented ADCC of NK cells in the presence of monocytes or neutrophils. The indirect effect of histamine on ADCC of NK cells and the effect of histamine on ADCC of monocytes/neutrophils were completely antagonized by the specific H2 receptor (H2R) blocker ranitidine. We conclude that activation of H2R suppresses ADCC reactivity of monocytes/neutrophils and, concomitantly, promotes ADCC reactivity of NK cells by abrogating a phagocyte-derived, suppressive signal.
- Subjects :
- Cell Communication
Cells, Cultured
Flow Cytometry
Granulocytes ultrastructure
Humans
Killer Cells, Natural ultrastructure
Monocytes ultrastructure
Ranitidine pharmacology
Receptors, Histamine H2 analysis
Receptors, Histamine H2 drug effects
Receptors, Histamine H2 physiology
Antibody-Dependent Cell Cytotoxicity physiology
Granulocytes cytology
Granulocytes physiology
Histamine physiology
Killer Cells, Natural cytology
Killer Cells, Natural physiology
Monocytes cytology
Monocytes physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0741-5400
- Volume :
- 55
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of leukocyte biology
- Publication Type :
- Academic Journal
- Accession number :
- 8120456
- Full Text :
- https://doi.org/10.1002/jlb.55.3.392