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Characteristics of voltage sensitive calcium channels in dendrites of cultured rat cerebellar neurons.
- Source :
-
Neuropharmacology [Neuropharmacology] 1993 Nov; Vol. 32 (11), pp. 1213-20. - Publication Year :
- 1993
-
Abstract
- We examined the effects of various pharmacological agents on the Ca2+ channels existing in the dendrites of cultured rat cerebellar neurons. The cultures consisted of Purkinje cells and non-Purkinje cells (deep cerebellar nuclear neurons and other non-granule neurons). Changes in intracellular free calcium concentration, [Ca2+]i, were monitored by digital imaging microfluorimetry using fura-2 as the indicator dye. In the Purkinje cell population increases in dendritic [Ca2+]i evoked by brief pulses of high K+ were very effectively blocked by (> 80%) by omega-Aga-IVA at low concentrations. Nimodipine and omega-conotoxins GVIA and MVIIC only blocked small components of the [Ca2+]i rise. In the non-Purkinje cells, omega-Aga-IVA was much less effective. omega-Conotoxin-GVIA blocked somewhat more and nimodipine blocked a similar percentage of the [Ca2+]i rise. omega-Conotoxin-MVIIC was quite ineffective in these cells. The results are discussed in terms of the types of voltage sensitive Ca2+ channels existing in the dendrites of these cells.
- Subjects :
- Animals
Calcium Channel Blockers pharmacology
Calcium Channels drug effects
Cells, Cultured
Cerebellum cytology
Cerebellum drug effects
Dendrites drug effects
Dendrites ultrastructure
Electrophysiology
Fluorometry
Neurons drug effects
Neurons ultrastructure
Purkinje Cells drug effects
Purkinje Cells metabolism
Rats
Calcium Channels metabolism
Cerebellum metabolism
Dendrites metabolism
Neurons metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0028-3908
- Volume :
- 32
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Neuropharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 8107975
- Full Text :
- https://doi.org/10.1016/0028-3908(93)90015-u