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Expression of gamma/delta T cell receptors in porcine thymus.
- Source :
-
Immunobiology [Immunobiology] 1993 Jun; Vol. 188 (1-2), pp. 70-81. - Publication Year :
- 1993
-
Abstract
- Swine possess an extraordinarily high number of T lymphocytes with the phenotype CD4-CD8- in peripheral blood as well as in lymphoid tissues. This subpopulation is subdivided into at least four subsets defined by the expression of CD2 and three biochemically distinct gamma/delta T cell receptors. The four subsets differ largely in their pattern of lymphoid homing in that CD2- subsets, historically referred to as null lymphocytes, predominate in the circulating pool, whereas CD2+ subsets are enriched in lymphoid tissues. Here we document the expression of all three types of gamma/delta T cell receptors by CD4-CD8- porcine thymocytes, which provides the first evidence for a thymic origination of all subsets of porcine gamma/delta T lymphocytes. The biochemical analysis shows that three distinct gamma-chains form disulfide-bonded cell surface heterodimers with a common delta-chain and that glycosylation of all chains is already completed within the thymus. Surprisingly, CD2- subsets, which are known to be enriched among thymic emigrants and which numerically predominate in peripheral blood, are underrepresented in the thymus, suggesting a high export rate.
- Subjects :
- Aging immunology
Animals
Antigens, Differentiation, T-Lymphocyte analysis
Antigens, Surface analysis
CD2 Antigens
CD4 Antigens analysis
CD8 Antigens analysis
Electrophoresis, Polyacrylamide Gel
Flow Cytometry
Phenotype
Receptors, Antigen, T-Cell, alpha-beta isolation & purification
Receptors, Immunologic analysis
Swine
T-Lymphocyte Subsets immunology
T-Lymphocyte Subsets metabolism
T-Lymphocytes immunology
Thymus Gland growth & development
Thymus Gland immunology
Receptors, Antigen, T-Cell, alpha-beta biosynthesis
T-Lymphocytes metabolism
Thymus Gland metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0171-2985
- Volume :
- 188
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Immunobiology
- Publication Type :
- Academic Journal
- Accession number :
- 8104881
- Full Text :
- https://doi.org/10.1016/s0171-2985(11)80488-2