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Recombinant capripoxvirus expressing the hemagglutinin protein gene of rinderpest virus: protection of cattle against rinderpest and lumpy skin disease viruses.
- Source :
-
Virology [Virology] 1994 Oct; Vol. 204 (1), pp. 425-9. - Publication Year :
- 1994
-
Abstract
- A cDNA clone containing the complete coding sequence of the hemagglutinin (H) protein gene of the RBOK vaccine strain of rinderpest virus, under the control of the vaccinia late promoter p11, was inserted by homologous recombination into the thymidine kinase gene of the KS-1 strain of capripoxvirus. The recombinant virus produced authentic H protein as judged by its electrophoretic mobility, transport to the cell surface of infected lamb testis cells, and reactivity with monoclonal antibodies specific for the H protein of rinderpest virus. The recombinant virus induced significant levels of rinderpest virus neutralizing antibodies in vaccinated cattle and protected them from clinical rinderpest after challenge with a lethal dose of a highly virulent heterologous strain of the virus. Protection was achieved using vaccine doses lower than those used with a similar recombinant expressing the fusion protein gene of rinderpest. The parental KS-1 virus is widely used as a vaccine against capripox viruses and so the rinderpest recombinant acts as a dual vaccine to protect cattle against both rinderpest and lumpy skin disease.
- Subjects :
- Animals
Antibodies, Viral blood
Capripoxvirus immunology
Cattle
Cell Line
Gene Expression
Genes, Viral genetics
Glycoproteins biosynthesis
Glycoproteins genetics
Hemagglutinins, Viral
Immunity, Active
Lumpy Skin Disease immunology
Membrane Proteins
Promoter Regions, Genetic genetics
Recombinant Fusion Proteins biosynthesis
Recombinant Fusion Proteins immunology
Rinderpest immunology
Rinderpest virus genetics
Thymidine Kinase genetics
Vaccination
Vaccines, Synthetic genetics
Vaccinia virus genetics
Viral Fusion Proteins genetics
Viral Fusion Proteins immunology
Viral Proteins biosynthesis
Viral Proteins genetics
Viral Structural Proteins genetics
Viral Vaccines genetics
Viral Vaccines immunology
Capripoxvirus genetics
Glycoproteins immunology
Lumpy Skin Disease prevention & control
Rinderpest prevention & control
Rinderpest virus immunology
Vaccines, Synthetic immunology
Viral Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0042-6822
- Volume :
- 204
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 8091673
- Full Text :
- https://doi.org/10.1006/viro.1994.1548