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Grain dust-induced airflow obstruction and inflammation of the lower respiratory tract.
- Source :
-
American journal of respiratory and critical care medicine [Am J Respir Crit Care Med] 1994 Sep; Vol. 150 (3), pp. 611-7. - Publication Year :
- 1994
-
Abstract
- To investigate the relationship between the physiologic and biologic effects of grain dust inhalation, we exposed 15 nonsmoking, nonasthmatic, nonatopic male grain handlers to buffered saline and aqueous corn dust extract by inhalation challenge in a crossover study. The inhalation challenges to buffered saline and corn dust extract were separated by at least 14 d. Compared with buffered saline, inhalation of corn dust extract resulted in significant airflow obstruction, which was observed within 30 min of exposure and persisted for 5 h. Inhalation of corn dust extract resulted in an acute inflammatory response characterized by higher concentrations of neutrophils (p = 0.001), IL-1 beta (p = 0.001), IL-1RA (p = 0.001), IL-6 (p = 0.001), IL-8 (p = 0.001), and TNF-alpha (p = 0.04) in bronchoalveolar lavage (BAL) fluid. mRNA levels specific for IL-1 beta, IL-1RA, IL-6, and IL-8 from cells present in the BAL fluid were significantly greater after challenge with corn dust extract than after challenge with buffered saline. Importantly, no significant differences were observed in the concentration of lymphocytes or eosinophils in the BAL fluid following inhalation of corn dust extract, and the concentrations of histamine and 15-HETE were similar in BAL fluid after the two challenges. The maximal percentage decrease in FEV1 was significantly associated with the absolute neutrophil concentration in the BAL fluid (p = 0.001), as well as the concentration of TNF-alpha (p = 0.03), IL-1 beta (p = 0.005), IL-1RA (p = 0.001), IL-6 (p = 0.001), and IL-8 (p = 0.001) in the BAL fluid.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- Adolescent
Adult
Agricultural Workers' Diseases diagnosis
Agricultural Workers' Diseases physiopathology
Airway Obstruction diagnosis
Airway Obstruction physiopathology
Allergens administration & dosage
Bronchoalveolar Lavage Fluid chemistry
Cytokines analysis
Humans
Inflammation diagnosis
Inflammation etiology
Inflammation physiopathology
Iowa
Male
Middle Aged
Nebulizers and Vaporizers
Polymerase Chain Reaction
RNA, Messenger analysis
Respiratory Tract Diseases diagnosis
Respiratory Tract Diseases physiopathology
Agricultural Workers' Diseases etiology
Airway Obstruction etiology
Dust adverse effects
Edible Grain adverse effects
Respiratory Tract Diseases etiology
Subjects
Details
- Language :
- English
- ISSN :
- 1073-449X
- Volume :
- 150
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- American journal of respiratory and critical care medicine
- Publication Type :
- Academic Journal
- Accession number :
- 8087327
- Full Text :
- https://doi.org/10.1164/ajrccm.150.3.8087327