Back to Search
Start Over
Identification of a unique group of human papillomavirus type 16 sequence variants among clinical isolates from Barbados.
- Source :
-
The Journal of general virology [J Gen Virol] 1994 Sep; Vol. 75 ( Pt 9), pp. 2457-62. - Publication Year :
- 1994
-
Abstract
- The naturally occurring sequence variation of human papillomavirus type 16 (HPV-16) was analysed by direct sequence analysis of the PCR products of the long control region (LCR), the E5 and E7 open reading frames (ORFs), a segment of the L2 ORF overlapping the early viral poly(A) signal and a small segment of the L1 ORF or clinical isolates from Barbados and The Netherlands. Despite the widely different geographical and ethnic origin of the two groups of specimens, sequence analysis revealed relatively few mutational differences. Analysis of the LCR and the E5 ORF appeared to be the minimum requirement for the correct positioning of these variants in the HPV-16 phylogenetic tree. Most of the Barbadian variants appeared to be located at a unique position in the HPV-16 phylogenetic tree, at the internal branch close to the point where the European and Asian branches diverge. In contrast, most of the Dutch samples were located on the European branch.
- Subjects :
- Asia
Barbados
Base Sequence
DNA Primers
Ethnicity
Europe
Humans
Molecular Sequence Data
Mutation
Netherlands
Oncogene Proteins, Viral genetics
Open Reading Frames
Papillomavirus E7 Proteins
Genetic Variation
Papillomaviridae genetics
Papillomaviridae isolation & purification
Phylogeny
Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1317
- Volume :
- 75 ( Pt 9)
- Database :
- MEDLINE
- Journal :
- The Journal of general virology
- Publication Type :
- Academic Journal
- Accession number :
- 8077948
- Full Text :
- https://doi.org/10.1099/0022-1317-75-9-2457