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Effect of calcitriol on growth, differentiation, chemokine mRNA expression of cultured keratinocytes and on keratinocyte-T cell binding.
- Source :
-
Acta dermato-venereologica. Supplementum [Acta Derm Venereol Suppl (Stockh)] 1994; Vol. 186, pp. 52-4. - Publication Year :
- 1994
-
Abstract
- Calcitriol has recently been shown to be effective against psoriasis. However, its mode of action is not exactly known. The present study focused on the influence of calcitriol on growth, differentiation, chemokine mRNA and ICAM-1 mRNA expression of keratinocytes (KC) and on the binding of T-cells to keratinocytes. In vitro studies showed that calcitriol has a strong anti-proliferative effect and induces terminal differentiation. gamma-IP-10 and ICAM-1 mRNA were induced by gamma-IFN, an induction not influenced by calcitriol. Moreover, the functional expression of ICAM-1 on the KC cell surface as measured by a cell adhesion assay, was not influenced either. IL-8 and huGRO mRNAs were constitutively produced in KC, as was demonstrated after incubation with cycloheximide. Up-regulation of both IL-8 and huGRO mRNA by IL-1 alpha was also not affected by calcitriol. It is concluded that calcitriol has a strong antiproliferative activity and does not interfere with KC responsiveness to gamma-IFN and IL-alpha induced chemokine expression or with the adhesion of T-cells to keratinocytes.
- Subjects :
- Cell Adhesion drug effects
Cell Differentiation drug effects
Cell Division drug effects
Cells, Cultured
Chemotactic Factors genetics
Cytokines genetics
Humans
Keratinocytes cytology
Keratinocytes metabolism
Keratinocytes physiology
RNA, Messenger metabolism
Calcitriol pharmacology
Keratinocytes drug effects
T-Lymphocytes physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0365-8341
- Volume :
- 186
- Database :
- MEDLINE
- Journal :
- Acta dermato-venereologica. Supplementum
- Publication Type :
- Academic Journal
- Accession number :
- 8073837