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Sequence and function of LuxO, a negative regulator of luminescence in Vibrio harveyi.
- Source :
-
Molecular microbiology [Mol Microbiol] 1994 May; Vol. 12 (3), pp. 403-12. - Publication Year :
- 1994
-
Abstract
- Density-dependent expression of luminescence in Vibrio harveyi is regulated by the concentration of extracellular signal molecules (autoinducers) in the culture medium. A recombinant clone that restored function to one class of spontaneous dim mutants was found to encode a function required for the density-dependent response. Transposon Tn5 insertions in the recombinant clone were isolated, and the mutations were transferred to the genome of V. harveyi for examination of mutant phenotypes. Expression of luminescence in V. harveyi strains with transposon insertions in one locus, luxO, was independent of the density of the culture and was similar in intensity to the maximal level observed in wild-type bacteria. Sequence analysis of luxO revealed one open reading frame that encoded a protein, LuxO, similar in amino acid sequence to the response regulator domain of the family of two-component, signal transduction proteins. The constitutive phenotype of LuxO- mutants indicates that LuxO acts negatively to control expression of luminescence, and relief of repression by LuxO in the wild type could result from interactions with other components in the Lux signalling system.
- Subjects :
- Amino Acid Sequence
Base Sequence
Gene Expression Regulation, Bacterial
Models, Genetic
Molecular Sequence Data
Mutagenesis, Insertional
Recombinant Proteins
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Signal Transduction genetics
Vibrio growth & development
Bacterial Proteins genetics
Genes, Bacterial genetics
Genes, Regulator genetics
Luminescent Measurements
Repressor Proteins genetics
Transcription Factors
Vibrio genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0950-382X
- Volume :
- 12
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Molecular microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 8065259
- Full Text :
- https://doi.org/10.1111/j.1365-2958.1994.tb01029.x