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Characterization of naturally acquired human IgG responses against the N-terminal region of the merozoite surface protein 1 of Plasmodium vivax.
- Source :
-
The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 1994 Jul; Vol. 51 (1), pp. 68-76. - Publication Year :
- 1994
-
Abstract
- The primary structure of the merozoite surface protein 1 of Plasmodium vivax (PvMSP-1) revealed the existence of conserved and polymorphic blocks of the protein among different Plasmodium species. To characterize the naturally acquired IgG antibody responses to the PvMSP-1 molecule, the entire N-terminal portion of this protein was expressed as 10 overlapping glutathione S-transferase fusion proteins. The affinity-purified recombinant products were tested by enzyme-linked immunosorbent assay and Western blot against the sera of malaria patients from the state of Rondonia, Brazil. We found that the majority of these sera did not contain IgG antibodies recognizing recombinant proteins expressing exclusively interspecies conserved blocks of the molecule. In contrast, a high proportion of these same sera reacted against recombinant products expressing interspecies polymorphic regions of this protein. The poor B cell immunogenicity of the interspecies conserved blocks of the PvMSP-1 molecule most likely reflects important and unknown structural or functional features of these regions.
- Subjects :
- Adult
Animals
Antigens, Surface immunology
Brazil epidemiology
Cloning, Molecular
DNA, Protozoan chemistry
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Female
Gene Expression Regulation
Humans
Immunoblotting
Malaria, Vivax epidemiology
Male
Merozoite Surface Protein 1
Plasmodium vivax genetics
Prevalence
Protein Precursors genetics
Protozoan Proteins genetics
Recombinant Fusion Proteins immunology
Antibodies, Protozoan biosynthesis
Antigens, Protozoan immunology
Immunoglobulin G biosynthesis
Malaria, Vivax immunology
Plasmodium vivax immunology
Protein Precursors immunology
Protozoan Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9637
- Volume :
- 51
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The American journal of tropical medicine and hygiene
- Publication Type :
- Academic Journal
- Accession number :
- 8059917
- Full Text :
- https://doi.org/10.4269/ajtmh.1994.51.68