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Molecular cloning of sheep lung dipeptidase: a glycosyl phosphatidylinositol-anchored ectoenzyme that converts leukotriene D4 to leukotriene E4.
- Source :
-
Biochimica et biophysica acta [Biochim Biophys Acta] 1994 Jul 18; Vol. 1226 (3), pp. 337-40. - Publication Year :
- 1994
-
Abstract
- Sheep lung dipeptidase was released from a plasma membrane preparation by digestion with a phosphatidylinositol-specific phospholipase C. The dipeptidase was purified to homogeneity using affinity chromatography followed by high performance liquid chromatography. The NH2-terminal sequence was determined and employed to prepare a probe to clone the c-DNA of the enzyme. The primary structure of sheep lung dipeptidase, deduced from the c-DNA exhibited a high homology to kidney dipeptidases cloned from other animal species. Northern analysis detected the m-RNA of the dipeptidase in lung, kidney, and intestinal tissues of the sheep.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Cell Membrane enzymology
Cloning, Molecular
DNA, Complementary genetics
Dipeptidases isolation & purification
Dipeptidases metabolism
Molecular Sequence Data
RNA, Messenger analysis
Sheep
Type C Phospholipases
Dipeptidases genetics
Leukotriene D4 metabolism
Leukotriene E4 metabolism
Lung enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 1226
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 8054366
- Full Text :
- https://doi.org/10.1016/0925-4439(94)90046-9