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Human cytotrophoblast invasion is up-regulated by epidermal growth factor: evidence that paracrine factors modify this process.
- Source :
-
Developmental biology [Dev Biol] 1994 Aug; Vol. 164 (2), pp. 550-61. - Publication Year :
- 1994
-
Abstract
- Formation of the human placenta requires a subset of cytotrophoblast stem cells to acquire an invasive phenotype. We examined the effect on cytotrophoblast invasiveness of growth factors that control the differentiation of other cells. Exogenous TGF-beta 1, PDGF-AA, PDGF-BB, and TNF-alpha affected neither cell morphology nor the rate of cytotrophoblast invasion in vitro. In contrast, addition of EGF to first trimester cytotrophoblast cultures produced dramatic changes in morphology and a severalfold increase in invasive capacity. The effects of EGF on later gestation cytotrophoblasts, whose invasive capacity is diminished, were much less pronounced. Next we investigated whether cytotrophoblasts themselves produce ligands that interact with the EGF receptor. A radioimmunoassay and a radioreceptor assay failed to detect EGF receptor ligands in cytotrophoblast-conditioned medium. Likewise, by RT-PCR cytotrophoblasts expressed neither EGF nor TGF-alpha mRNA. In contrast, EGF receptor mRNA was expressed and its protein levels remained constant during the experiment. Immunolocalization using F(ab') fragments of an anti-human EGF antibody failed to detect this growth factor in the chorionic villus. We conclude that maternal ligands that interact with the EGF receptor could play an important role by up-regulating trophoblast invasion, particularly during the early stages of pregnancy.
- Subjects :
- Becaplermin
Cell Differentiation
Female
Humans
Immunohistochemistry
Platelet-Derived Growth Factor physiology
Polymerase Chain Reaction
Pregnancy
Proto-Oncogene Proteins c-sis
Transforming Growth Factor alpha physiology
Transforming Growth Factor beta physiology
Trophoblasts cytology
Up-Regulation
Epidermal Growth Factor physiology
Growth Substances physiology
Trophoblasts physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0012-1606
- Volume :
- 164
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Developmental biology
- Publication Type :
- Academic Journal
- Accession number :
- 8045351
- Full Text :
- https://doi.org/10.1006/dbio.1994.1223