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Origin of circulating acute phase cytokines: modified proteins may trigger IL-6 production by macrophages. Preliminary report.
- Source :
-
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society [J Physiol Pharmacol] 1994 Mar; Vol. 45 (1), pp. 69-80. - Publication Year :
- 1994
-
Abstract
- Human peripheral blood monocytes isolated by centrifugation with Mono-Poly resolving medium, and human alveolar macrophages obtained by lung lavage during fiberoscopic bronchoscopy, were cultured in RPMI containing 2% foetal calf serum. The cultures were exposed to modified human proteins: alpha-1-antitrypsin cleaved with papain, fibrinogen degradation products (fraction D) purified from plasmin digest, and non-enzymatically glycosylated (glycated) serum albumin. Conditioned macrophage media were tested for the contents of acute phase cytokines by bioassay with hepatoma cells, and the concentration of interleukin-6 was determined with ELISA. Modified proteins stimulated macrophages to produce acute phase cytokines and the response was not abrogated by polymyxin B in distinction to stimulation of macrophages by endotoxin. Our data indicate that some proteolytically damaged proteins or the end glycosylation products formed in pathological states (acute inflammation, diabetes) may be responsible for the appearance of cytokines in the circulation.
- Subjects :
- Amino Acid Sequence
Animals
Enzyme-Linked Immunosorbent Assay
Escherichia coli metabolism
Fibrinogen pharmacology
Humans
In Vitro Techniques
Lipopolysaccharides antagonists & inhibitors
Lipopolysaccharides pharmacology
Liver Neoplasms, Experimental metabolism
Molecular Sequence Data
Monocytes metabolism
Polymyxin B pharmacology
Rats
Serum Albumin chemistry
Serum Albumin pharmacology
Tumor Cells, Cultured
alpha 1-Antitrypsin pharmacology
Interleukin-6 biosynthesis
Macrophages, Alveolar metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0867-5910
- Volume :
- 45
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of physiology and pharmacology : an official journal of the Polish Physiological Society
- Publication Type :
- Academic Journal
- Accession number :
- 8043910