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Flow cytometric DNA analysis of squamous cell carcinomas of the oral cavity: correlation with clinical and histopathological features.

Flow cytometric DNA analysis of squamous cell carcinomas of the oral cavity: correlation with clinical and histopathological features.

Authors :
Balsara BR
Borges AM
Pradhan SA
Rajpal RM
Bhisey AN
Source :
European journal of cancer. Part B, Oral oncology [Eur J Cancer B Oral Oncol] 1994; Vol. 30B (2), pp. 98-101.
Publication Year :
1994

Abstract

DNA content was measured in 68 squamous cell carcinomas (SCC) of the oral cavity by flow cytometry. Samples fixed in 95% alcohol and disaggregated with 0.5% pepsin were stained with 4,6-diamidino-phenylindole (DAPI) for flow cytometry. The tumours were classified according to the TNM classification--1987, and graded histopathologically. A positive correlation between tumour size and ploidy status was observed. Poorly differentiated tumours were mainly non-diploid (P < 0.01, chi 2). A majority of the node positive (N+) tumours were non-diploid (P < 0.05). It was possible to distinguish diploid, N+, T4 tumours from diploid, N-, T4 tumours by their higher S-phase fraction (SpF). SpF was also a useful parameter to differentiate diploid, N+ tumours from diploid, N- tumours among the moderately differentiated (MD) SCC. These results suggest that ploidy and SpF can be useful correlates of tumour behaviour.

Details

Language :
English
ISSN :
0964-1955
Volume :
30B
Issue :
2
Database :
MEDLINE
Journal :
European journal of cancer. Part B, Oral oncology
Publication Type :
Academic Journal
Accession number :
8032308
Full Text :
https://doi.org/10.1016/0964-1955(94)90060-4