Back to Search
Start Over
Expression of stromelysin-3 and nm23 in breast carcinoma and related tissues.
- Source :
-
International journal of cancer [Int J Cancer] 1994 Jul 15; Vol. 58 (2), pp. 157-60. - Publication Year :
- 1994
-
Abstract
- Biological functions proposed for the ST3 and nm23 genes in tumour development and progression seem to be directly opposed. Stromelysin-3 (ST3) is a putative member of the matrix metalloproteinase family. ST3 has been implicated in the progression of epithelial malignancies, specifically with regard to an invasive (and therefore potentially metastasizing) phenotype. The nm23 gene, on the other hand, encodes a nucleoside diphosphate kinase which allegedly has a metastasis-suppressor-type function. It was therefore of interest to compare the expression of ST3 and nm23 in various surgically excised normal and neoplastic breast tissues. RNA was isolated from over 200 surgical specimens and studied by Northern blots. Normal breast tissues did not express ST3, and ST3 expression was detected in only 1 of 20 normal axillary lymph nodes. None of 7 fibroadenomas expressed ST3. In contrast, 60% of primary and metastatic breast carcinomas contained ST3-mRNA. The expression of ST3 was mainly confined to invasive carcinomas and was observed less frequently in pure ductal carcinoma in situ (DCIS) lesions. Our results support the suggestion that ST3 expression is related to the malignant process in breast cancer. The role of nm23 is far less clear-cut.
- Subjects :
- Biopsy
Blotting, Northern
Breast chemistry
Breast Neoplasms chemistry
Breast Neoplasms pathology
Carcinoma in Situ chemistry
Carcinoma in Situ genetics
Carcinoma in Situ pathology
Carcinoma, Ductal, Breast chemistry
Carcinoma, Ductal, Breast genetics
Carcinoma, Ductal, Breast pathology
Female
Gene Expression
Humans
Lymphatic Metastasis
Matrix Metalloproteinase 11
Metalloendopeptidases analysis
NM23 Nucleoside Diphosphate Kinases
Neoplasm Invasiveness
RNA, Messenger analysis
RNA, Messenger genetics
Transcription Factors analysis
Breast physiology
Breast Neoplasms genetics
Metalloendopeptidases genetics
Monomeric GTP-Binding Proteins
Nucleoside-Diphosphate Kinase
Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0020-7136
- Volume :
- 58
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 8026874
- Full Text :
- https://doi.org/10.1002/ijc.2910580202