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Monoclonal antibodies against metalloporphyrins. Specificity of interaction with structurally different metalloporphyrins.

Authors :
Savitsky AP
Demcheva MV
Mantrova EYu
Ponomarev GV
Source :
FEBS letters [FEBS Lett] 1994 Dec 05; Vol. 355 (3), pp. 314-6.
Publication Year :
1994

Abstract

Monoclonal antibodies against Pd-coproporphyrin I have been obtained. The antibody specificity for free as well as for conjugated Pd-coproporphyrin I is characterized. Affinity constants are estimated for 3 monoclonal antibodies effectively interacting with free Pd-coproporphyrin I. A comparative study on the binding of monoclonal antibodies with analogues and derivatives of Pd-coproporphyrin I has revealed that the antigen is mainly located inside the antibody paratope. The protein adjoins complementary to the metalloporphyrin in such a manner that antibodies obtained discern only isomer I, and to some degree, isomer III of coproporphyrin.

Details

Language :
English
ISSN :
0014-5793
Volume :
355
Issue :
3
Database :
MEDLINE
Journal :
FEBS letters
Publication Type :
Academic Journal
Accession number :
7988696
Full Text :
https://doi.org/10.1016/0014-5793(94)01221-0