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New cell line from hairy-cell leukaemia producing interleukin-6 after Epstein-Barr virus immortalization.
- Source :
-
Acta haematologica [Acta Haematol] 1994; Vol. 92 (1), pp. 8-13. - Publication Year :
- 1994
-
Abstract
- A hairy-cell leukaemia (HCL) cell line, HCL-O, was established from the peripheral blood of a 62-year-old Japanese patient with a unique variant of HCL strongly expressing CD21, the receptor for the Epstein-Barr virus (EBV). The HCL-O cells expressed antigens similar and dissimilar to those expressed with the original hairy cells. The HCL-O cells were more mature than the original cells in their degree of B-cell differentiation, as indicated by a decrease of CD19 and surface immunoglobulin (sIg) expression together with the appearance of CD38 and cytoplasmic Ig (cIg). In addition, the cells expressed CD11c recognized by Leu-M5, a monoclonal antibody usually positive for HCL. Their karyotype and Ig gene rearrangement pattern were identical to those of the original cells. The EBV genome was detected in the HCL-O cells but not in the original cells. The HCL-O cells spontaneously produced a large quantity of interleukin-6 (IL-6) in the conditioned medium, whereas IL-6 serum level was not so high. These findings indicate that the HCL-O cell line is derived from the leukaemic hairy cells and possibly, in vitro EBV infection took place easily in the original hairy cells through their CD21, resulting in subsequent immortalization. IL-6 production by HCL-O cells may be induced or enhanced by EBV, and the secreted IL-6 might play a role in their own growth or differentiation.
- Subjects :
- Cytological Techniques
Gene Rearrangement genetics
Genes, Immunoglobulin genetics
Humans
Japan
Karyotyping
Leukemia, Hairy Cell genetics
Leukocytosis blood
Leukocytosis genetics
Male
Middle Aged
Receptors, Complement 3d analysis
Tumor Cells, Cultured
Cell Transformation, Viral
Herpesvirus 4, Human
Interleukin-6 blood
Leukemia, Hairy Cell blood
Subjects
Details
- Language :
- English
- ISSN :
- 0001-5792
- Volume :
- 92
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Acta haematologica
- Publication Type :
- Academic Journal
- Accession number :
- 7985490
- Full Text :
- https://doi.org/10.1159/000204129