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Hepatitis C virus genotypes in France: comparison of clinical features of patients infected with HCV type I and type II.
- Source :
-
Journal of hepatology [J Hepatol] 1994 Jul; Vol. 21 (1), pp. 70-5. - Publication Year :
- 1994
-
Abstract
- Two major hepatitis C virus genotypes, F1 and F2, corresponding to hepatitis C virus type I and type II respectively, were found in France. To investigate the correlation between infection with these genotypes (F1 and F2) and clinical features of patients, serum samples proven to be hepatitis C virus positive by polymerase chain reaction amplification on 5' non-coding region were further amplified in the NS3 region with nested polymerase chain reaction. The NS3-polymerase chain reaction products were Southern blotted and hybridized with specific probes to identify the genotype of hepatitis C virus. Of 70 samples 64 were NS3-polymerase chain reaction positive. Twenty-eight (40%) samples were hepatitis C virus type I (F1) and 34 (49%) were hepatitis C virus type II (F2), while one sample (HB) hybridized with both probes and another (HN) hybridized with neither. Some samples were sequenced, with results consistent with those of hybridization. The HB sample was related more to hepatitis C virus type II than to type I and the HN sample was divergent from both type I and type II genotypes. Clinical profiles of patients infected with hepatitis C virus type I and type II were compared. Type I infected patients were younger (p < 0.01) and more often male (p < 0.05) than those of the type II group. Nine of 28 patients in the type I infected group had a history of drug abuse, whereas none did in the type II group. Five of 22 (23%) type I infected patients and 19 of 32 (59%) type II infected patients had cirrhosis (p < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- Adult
Aged
Base Sequence
Cloning, Molecular
DNA Primers
Female
France
Genotype
Hepacivirus classification
Hepacivirus isolation & purification
Hepatitis C pathology
Hepatitis C therapy
Humans
Interferon-alpha therapeutic use
Liver pathology
Liver virology
Male
Middle Aged
Molecular Sequence Data
Polymerase Chain Reaction methods
Hepacivirus genetics
Hepatitis C virology
Subjects
Details
- Language :
- English
- ISSN :
- 0168-8278
- Volume :
- 21
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of hepatology
- Publication Type :
- Academic Journal
- Accession number :
- 7963424
- Full Text :
- https://doi.org/10.1016/s0168-8278(94)80139-8