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Lipase and acidic phosphatase from the psychrotrophic bacterium Pseudomonas fluorescens: two enzymes whose synthesis is regulated by the growth temperature.
- Source :
-
FEMS microbiology letters [FEMS Microbiol Lett] 1994 Sep 15; Vol. 122 (1-2), pp. 13-8. - Publication Year :
- 1994
-
Abstract
- In the psychrotrophic bacterium Pseudomonas fluorescens, the production of several enzymes that otherwise differ in their cell location, growth phase production and inducibility appeared to be similarly regulated by the growth temperature. In order to determine the level of this regulation, the expression of the apo and lip genes encoding two of these enzymes, the acidic phosphatase and lipase, respectively, was studied at different temperatures. Both genes were optimally expressed at 17.5 degrees C, i.e., at the optimal temperature of production of the enzymes; however, the low level of activity at the highest temperature could be due to an additional post-transcriptional control.
- Subjects :
- Acid Phosphatase genetics
Escherichia coli genetics
Gene Expression Regulation, Enzymologic
Lipase genetics
Pseudomonas fluorescens genetics
Pseudomonas fluorescens growth & development
RNA, Messenger metabolism
Transformation, Bacterial
beta-Galactosidase metabolism
Acid Phosphatase biosynthesis
Lipase biosynthesis
Pseudomonas fluorescens enzymology
Temperature
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1097
- Volume :
- 122
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- FEMS microbiology letters
- Publication Type :
- Academic Journal
- Accession number :
- 7958764
- Full Text :
- https://doi.org/10.1111/j.1574-6968.1994.tb07136.x