Back to Search
Start Over
Chicken oocyte growth is mediated by an eight ligand binding repeat member of the LDL receptor family.
- Source :
-
The EMBO journal [EMBO J] 1994 Nov 01; Vol. 13 (21), pp. 5165-75. - Publication Year :
- 1994
-
Abstract
- Deposition of the yolk mass components of chicken oocytes, very low density lipoprotein (VLDL) and vitellogenin (VTG), is mediated by a 95 kDa plasma membrane protein, termed VLDL/VTG receptor (VLDL/VTGR). Molecular characterization of the VLDL/VTGR revealed that it is a member of the LDLR gene superfamily, and harbours eight complement-type, cysteine-rich ligand binding repeats at the N-terminus. This ligand binding domain structure is the hallmark of the recently discovered mammalian so-called VLDLRs, whose true physiological function remains to be elucidated. Northern blot analysis revealed that this receptor is expressed almost exclusively in oocytes, with very much lower levels of hybridizing transcripts present in heart and skeletal muscle. Heterologous expression of the cloned receptor demonstrated its ability to bind both VLDL and VTG. The receptor gene is located on the avian sex chromosome Z, in agreement with the sex linkage of a single-gene defect in animals that fail to reproduce because of the lack of expression of functional VLDL/VTGR. In situ hybridization analysis of oocytes suggested that VLDL/VTGR mRNA may relocalize during oocyte growth. Thus, the current study has identified and characterized the first non-mammalian VLDLR. Its key role in avian reproduction and extremely high evolutionary conservation shed new light on VLDLR function in mammals, which also express the gene in ovaries.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Binding Sites genetics
Cell Membrane chemistry
Chromosome Mapping
DNA, Complementary genetics
Female
In Situ Hybridization
Lipoproteins, VLDL metabolism
Male
Molecular Sequence Data
Ovary ultrastructure
Protein Conformation
Receptors, Cell Surface biosynthesis
Receptors, Cell Surface metabolism
Receptors, LDL biosynthesis
Receptors, LDL metabolism
Recombinant Proteins biosynthesis
Repetitive Sequences, Nucleic Acid genetics
Sequence Homology, Amino Acid
Sex Chromosomes
Tissue Distribution
Vitellogenins metabolism
Chickens physiology
Egg Proteins
Multigene Family genetics
Oocytes growth & development
Receptors, Cell Surface genetics
Receptors, LDL genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0261-4189
- Volume :
- 13
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- The EMBO journal
- Publication Type :
- Academic Journal
- Accession number :
- 7957081
- Full Text :
- https://doi.org/10.1002/j.1460-2075.1994.tb06847.x