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Developmental regulation and tissue-specific expression of hamster dolichol-P-dependent N-acetylglucosamine-1-P transferase (GPT).
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1994 Oct 14; Vol. 204 (1), pp. 284-91. - Publication Year :
- 1994
-
Abstract
- The first enzyme in the dolichol pathway of protein N-glycosylation, dolichol-P-dependent N-acetylglucosamine-1-phosphate transferase, GPT, has been implicated in the development of a wide variety of eukaryotes. GPT is encoded by ALG7, an early growth-response gene, whose expression has been shown to affect the extent of N-glycosylation and secretion of proteins. To initiate the molecular characterization of ALG7 involvement in mammalian growth and differentiation, we have used the postnatally developing hamster submandibular gland (SMG) as an experimental paradigm. In this study we report that the ALG7 gene was differentially expressed during postnatal development and in terminally differentiated adult tissues. Throughout development, GPT activity paralleled ALG7 mRNA levels, suggesting that the amount of functional enzyme was determined by modulation of transcript abundance.
- Subjects :
- Animals
Animals, Newborn
Cell Differentiation
Cricetinae
Female
Intracellular Membranes enzymology
Microsomes enzymology
Organ Specificity
Pregnancy
RNA, Messenger analysis
RNA, Messenger biosynthesis
Submandibular Gland enzymology
Submandibular Gland growth & development
Transcription, Genetic
Transferases (Other Substituted Phosphate Groups) metabolism
Aging metabolism
Gene Expression Regulation, Enzymologic
Transferases (Other Substituted Phosphate Groups) biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 204
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 7945372
- Full Text :
- https://doi.org/10.1006/bbrc.1994.2457