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Nucleotide sequence of Nilaparvata lugens reovirus genome segment S8 coding for the major outer capsid protein.
- Source :
-
The Journal of general virology [J Gen Virol] 1994 Oct; Vol. 75 ( Pt 10), pp. 2803-6. - Publication Year :
- 1994
-
Abstract
- The complete nucleotide sequence of genome segment 8 (S8) of Nilaparvata lugens reovirus (NLRV) was determined. It consisted of 1802 nucleotides containing a long open reading frame (562 amino acids), which was expressed in Escherichia coli as a fusion protein. The expressed S8 product, a 62K protein, was detected by Western blotting using IgG directed against intact NLRV particles. This result indicates that S8 encodes the major outer capsid protein of NLRV. The protein exhibited 18.6% amino acid sequence identity with the predicted translation product of S10 of rice black-streaked dwarf virus.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Capsid biosynthesis
Capsid chemistry
Cloning, Molecular
Escherichia coli
Molecular Sequence Data
Open Reading Frames
Protein Biosynthesis
Protein Conformation
Recombinant Fusion Proteins biosynthesis
Recombinant Fusion Proteins chemistry
Sequence Homology, Amino Acid
Capsid genetics
Genome, Viral
Insecta virology
Reoviridae genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1317
- Volume :
- 75 ( Pt 10)
- Database :
- MEDLINE
- Journal :
- The Journal of general virology
- Publication Type :
- Academic Journal
- Accession number :
- 7931170
- Full Text :
- https://doi.org/10.1099/0022-1317-75-10-2803