Back to Search
Start Over
The helix-loop-helix containing transcription factor USF activates the promoter of the CD2 gene.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 1994 Oct 21; Vol. 269 (42), pp. 26525-30. - Publication Year :
- 1994
-
Abstract
- T cell development within the thymus involves the ordered expression of a number of tissue-specific components such as the CD2 gene. Control of expression of this gene is regulated by a well characterized 3' enhancer together with a promoter and upstream elements. The CD2 promoter is typical of a group of T cell-specific promoters that lack a TATA box and use multiple sites for initiation of transcription. An "E box" motif CACGTG, located just upstream from the most 5' initiation start site, was found to contribute a major effect to the level of basal transcription of a reporter gene. Analysis of the proteins in T cell extracts that bound to this site revealed that the bHLH-LZ protein USF was the major component. A functional role for USF was established in transient transfection experiments. Thus, this protein restored full promoter activity following repression caused by cotransfection with the E box binding bHLH-LZ protein Max. Taken together, these results indicate that an E box motif is critical to expression of the CD2 gene during T cell development and that the HLH protein USF acts as a transcriptional activator of the CD2 promoter.
- Subjects :
- Base Sequence
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Basic-Leucine Zipper Transcription Factors
Cells, Cultured
DNA-Binding Proteins metabolism
Humans
Molecular Sequence Data
Transcription, Genetic
Transcriptional Activation
Upstream Stimulatory Factors
CD2 Antigens genetics
Helix-Loop-Helix Motifs
Promoter Regions, Genetic
Transcription Factors physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 269
- Issue :
- 42
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 7929376