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Expression of the extracellular matrix molecule thrombospondin inversely correlates with malignant progression in melanoma, lung and breast carcinoma cell lines.
- Source :
-
International journal of cancer [Int J Cancer] 1994 Oct 15; Vol. 59 (2), pp. 191-5. - Publication Year :
- 1994
-
Abstract
- Thrombospondin (TSP) is a member of a family of extracellular matrix glycoproteins that may participate in multiple aspects of the metastatic cascade. We report an inverse correlation of steady-state Thbs-1 mRNA and protein expression with malignant progression among murine melanoma and human lung and breast carcinoma cell lines. Murine K-1735 melanoma cell lines of low metastatic potential, including K-1735 lines transfected with the murine nm23-1 cDNA, expressed higher TSP levels than related highly metastatic lines. In a model system of lung carcinoma malignant progression, immortalized human bronchial epithelial cells expressed higher TSP levels than v-Ki-ras, v-Ha-ras or n-ras transfectants, which in turn expressed higher TSP levels than tumor-derived, more aggressive variants. Among 3 unrelated breast carcinoma cell lines, Thbs-1 steady-state mRNA levels were greater in the 2 non-metastatic lines than the metastatic line. Our data show that malignant progression in some cell lines is associated with reduced TSP expression. The suppressive effects of nm23-1 transfection on metastatic potential are also associated with increased TSP expression; ras transfection, which results in increased tumorigenesis, is associated with decreased TSP expression.
- Subjects :
- Animals
Breast Neoplasms genetics
Cell Adhesion Molecules genetics
DNA Probes
Gene Expression
Genes, ras
Humans
Melanoma genetics
Membrane Glycoproteins genetics
Mice
Models, Biological
NM23 Nucleoside Diphosphate Kinases
Thrombospondins
Transcription Factors genetics
Transfection
Tumor Cells, Cultured
Breast Neoplasms metabolism
Breast Neoplasms pathology
Cell Adhesion Molecules biosynthesis
Melanoma metabolism
Melanoma pathology
Membrane Glycoproteins biosynthesis
Monomeric GTP-Binding Proteins
Nucleoside-Diphosphate Kinase
Subjects
Details
- Language :
- English
- ISSN :
- 0020-7136
- Volume :
- 59
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 7927918
- Full Text :
- https://doi.org/10.1002/ijc.2910590209