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Histamine receptors on bovine peripheral blood lymphocytes.
- Source :
-
The Journal of veterinary medical science [J Vet Med Sci] 1994 Feb; Vol. 56 (1), pp. 45-9. - Publication Year :
- 1994
-
Abstract
- Histamine receptors on bovine peripheral blood lymphocytes (PBL) were detected by three different methods: a rosetting technique, binding to histamine-bearing Sepharose beads and immunofluorescence staining. The rosetting technique used histamine-rabbit serum albumin (H-RSA) conjugated to bovine red blood cells to detect histamine receptors and this showed that 10.8% of bovine PBL were positive. A method using H-RSA conjugate coupled Sepharose beads also detected histamine receptor bearing PBL but was not quantitative. The indirect immunofluorescence method, by which the subpopulation of histamine receptor bearing lymphocytes can be easily double stained to concurrently identify the B cell marker, revealed that PBL, the B cell and T cell fraction of bovine PBL contained 18.4, 52.8 and 9.3% histamine receptor bearing cells, respectively. This method was found to be more stable and more easily quantifiable than the other two methods. Blocking tests using the histamine H1 receptor antagonist diphenhydramine and the histamine H2 receptor antagonist cimetidine suggested that bovine PBL have both H1 and H2 receptors on their surfaces. Addition of histamine into cultures of PBL at the concentration range 10(-6) to 10(-3) M suppressed the response of PBL to the mitogen phytohemagglutinin. The histamine induced suppression of mitogenesis could be reduced partially by the H2 receptor antagonist cimetidine, but not by the H1 antagonist diphenhydramine. It is possible that histamine induced suppression of PBL mitogenesis was mediated by H2 receptors on T cells.
- Subjects :
- Animals
B-Lymphocytes physiology
Cattle
Cells, Cultured
Female
Histamine pharmacology
Histamine H1 Antagonists pharmacology
Histamine H2 Antagonists pharmacology
Lymphocyte Activation drug effects
Lymphocytes drug effects
Lymphocytes immunology
Receptors, Histamine H1 analysis
Receptors, Histamine H1 drug effects
Receptors, Histamine H2 analysis
Receptors, Histamine H2 drug effects
Rosette Formation
T-Lymphocytes physiology
Lymphocytes physiology
Receptors, Histamine H1 physiology
Receptors, Histamine H2 physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0916-7250
- Volume :
- 56
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of veterinary medical science
- Publication Type :
- Academic Journal
- Accession number :
- 7911336
- Full Text :
- https://doi.org/10.1292/jvms.56.45