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Effects of pertussis and cholera toxin on the interferon-gamma stimulated immunocytochemical staining of ICAM-1 and inositol phosphate formation in a human renal carcinoma cell line.
- Source :
-
Virchows Archiv. B, Cell pathology including molecular pathology [Virchows Arch B Cell Pathol Incl Mol Pathol] 1993; Vol. 64 (6), pp. 345-50. - Publication Year :
- 1993
-
Abstract
- We have recently shown that interferon-gamma (IFN-gamma) stimulated immunocytochemical staining of the intercellular adhesion molecule ICAM-1 may be dependent on inositol phosphate formation in the human renal carcinoma cell line CaKi-1. In the present study we investigated the possible role of GTP-binding proteins (G-proteins) during IFN-gamma signalling. Preincubation of CaKi-1 cells for 24 h with increasing amounts of pertussis toxin (PT) or cholera toxin (CT), two regulators of G-protein activity, inhibited IFN-gamma induced ICAM-1 staining. Preincubation with PT or CT for 24 h also inhibited IFN-gamma induced inositol 1-monophosphate (Ins 1-P), inositol 1,4 bisphosphate (Ins 1,4-P2) and inositol 1,4,5 trisphosphate (Ins 1,4,5-P3) formation. Our findings suggest that IFN-gamma induced ICAM-1 staining and inositol phosphate formation in CaKi-1 cells is dependent on a PT and CT sensitive signalling pathway. This may reflect a role for G-proteins in the coupling of IFN-gamma receptor activation and phospholipase C catalyzed phosphoinositide hydrolysis.
- Subjects :
- Cell Adhesion Molecules analysis
Humans
Immunoenzyme Techniques
Intercellular Adhesion Molecule-1
Kidney Neoplasms immunology
Tumor Cells, Cultured
Carcinoma, Renal Cell immunology
Cell Adhesion Molecules drug effects
Cholera Toxin pharmacology
Inositol Phosphates biosynthesis
Interferon-gamma physiology
Pertussis Toxin
Virulence Factors, Bordetella pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0340-6075
- Volume :
- 64
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Virchows Archiv. B, Cell pathology including molecular pathology
- Publication Type :
- Academic Journal
- Accession number :
- 7908588
- Full Text :
- https://doi.org/10.1007/BF02915133