Relation between the trans-Golgi network and the Golgi stack on development of the Golgi apparatus of the ameloblast in developing rat molar tooth germs.

Background: The problem of how the functional compartments of the Golgi apparatus organizes during cell differentiation to become a well-formed Golgi apparatus is as yet an unresolved issue. This study was designed to define the involvement of the trans-Golgi network (TGN) and the Golgi stack in organizing the Golgi apparatus.
Methods: The distribution of the TGN marker enzyme was examined in the ameloblast of developing rat molar tooth germs using cytochemistry with Co-enzyme A phosphatase (CoA Pase) and cytidine monophosphatase (CMPase).
Results: Typically formed Golgi apparatus was observed in the secretory ameloblast but not in the presecretory ameloblast. Organization of the Golgi apparatus through the presecretory ameloblast was noted. In the presecretory ameloblast, Golgi stacks of different sizes and clusters of small vesicles were located in the cytoplasm lateral to the nucleus. The saccules with enzymes marked for TGN were also observed in the cytoplasm lateral to the nucleus. These saccules were adjacent to the cluster of small vesicles and/or the Golgi stack. Upon cell differentiation, Golgi stacks were seen in line along the long axis of the cell, and the file of the stacks in the cytoplasm lateral to the nucleus was formed. The positive saccule was seen in a parallel line equal to the length of the Golgi stacks.
Conclusions: In organizing the Golgi apparatus, the development process of the TGN and the Golgi stack appear to be different, and new Golgi stacks seem to be formed through the accumulation of small vesicles near the pre-existing TGN.