Serological typing of red clover necrotic mosaic virus isolates by enzyme-linked immunosorbent assay.

Authors :: Gallo J
Musil M
Source :: Acta virologica [Acta Virol] 1994 Aug; Vol. 38 (4), pp. 229-30.
Publication Year :: 1994
Abstract: Enzyme-linked immunosorbent assay (ELISA) enabled a more precise serological typing of isolates of red clover necrotic mosaic virus (RCNMV) originating from Slovak and Czech Republics, Poland, Sweden and Great Britain. We found 5 isolates of serotype A, 14 isolates of serotype B, and 6 isolates of serotype C. Some isolates represented mixtures of serotypes, namely A+B (1 isolate), A+C (1), B+C (7), and A+B+C (5). ELISA was found to be a more suitable method of serotype identification of RCNMV isolates than the double diffusion agar gel test for its higher sensitivity and greater selectivity.

Subjects :: Antibodies, Viral
Enzyme-Linked Immunosorbent Assay statistics & numerical data
Immunoglobulin G
Mosaic Viruses immunology
Mosaic Viruses isolation & purification
Sensitivity and Specificity
Serotyping statistics & numerical data
Virology statistics & numerical data
Enzyme-Linked Immunosorbent Assay methods
Mosaic Viruses classification
Serotyping methods
Virology methods

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