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Natural occurrence and characterization of the Lyme disease spirochete, Borrelia burgdorferi, in cotton rats (Sigmodon hispidus) from Georgia and Florida.
- Source :
-
The Journal of parasitology [J Parasitol] 1995 Feb; Vol. 81 (1), pp. 30-6. - Publication Year :
- 1995
-
Abstract
- This is the first report of natural infection by Borrelia burgdorferi in the cotton rat Sigmodon hispidus. Nine B. burgdorferi isolates were obtained from ear tissues, urinary bladders, or both, by culturing tissues in BSKII medium. The rat from which the SI-3 isolate was cultured was from the same site (Sapelo Island, Georgia) as an infected cotton mouse Peromyscus gossypinus and Ixodes scapularis tick reported previously. The 8 B. burgdorferi isolates from rats in Florida included 1 (AI-1) from Amelia Island, 1 (FD-1) from Faver-Dykes State Park, and 6 (MI-3 through MI-8) from Merritt Island. The distance between Sapelo Island and Merritt Island is approximately 400 km. All B. burgdorferi isolates were characterized by indirect immunofluorescence using monoclonal antibodies to OspA (H3TS, H5332) and OspB (H5TS, H6831), polymerase chain reaction detection of specific B. burgdorferi B-31 DNA target sequences (ospA, fla, and a random chromosomal sequence), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of spirochetal proteins. The phenotypic and genotypic characteristics of the isolates are discussed, as well as the probable importance of the cotton rat as a reservoir for B. burgdorferi in the southern United States.
- Subjects :
- Animals
Antibodies, Monoclonal immunology
Antigens, Bacterial immunology
Antigens, Surface genetics
Bacterial Outer Membrane Proteins genetics
Bacterial Vaccines
Base Sequence
Borrelia burgdorferi Group genetics
Borrelia burgdorferi Group immunology
DNA Primers chemistry
Densitometry
Disease Reservoirs
Electrophoresis, Agar Gel
Electrophoresis, Polyacrylamide Gel
Flagellin genetics
Florida epidemiology
Fluorescent Antibody Technique
Genes, Bacterial
Georgia epidemiology
Lyme Disease epidemiology
Molecular Sequence Data
Polymerase Chain Reaction
Borrelia burgdorferi
Borrelia burgdorferi Group isolation & purification
DNA, Bacterial analysis
Lipoproteins
Lyme Disease veterinary
Rodent Diseases epidemiology
Sigmodontinae microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3395
- Volume :
- 81
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 7876974