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The mature form of interleukin-1 alpha is constitutively expressed in immature male germ cells from rat.
- Source :
-
Molecular and cellular endocrinology [Mol Cell Endocrinol] 1994 Nov; Vol. 105 (2), pp. R19-23. - Publication Year :
- 1994
-
Abstract
- In the present study we show that immature germ cells from rat testis contain both interleukin-1 alpha (IL-1 alpha) mRNA and immunoreactive proteins. In contrast, in primary cultures of Sertoli cells and peritubular cells. IL-1 alpha mRNA and immunoreactive protein were below the levels of detection. Immunoblots of lysates from these germ cells showed the presence of strong 17 kDa bands (mature forms of IL-1 alpha) and a much weaker 33 kDa band (precursor form). The finding of cell-associated mature forms of IL-1 alpha in germ cells indicates that immature male germ cells are able to process IL-1 alpha independent of its secretion. Data from isolated cell fractions, as well as from whole testis tissue from rats of various ages, indicate that IL-1 alpha expression takes place in late pachytene spermatocytes and early round spermatids. Whether IL-1 alpha plays a role intracellularly in germ cells or exerts its effects on neighboring Sertoli cells remains to be shown.
- Subjects :
- Animals
Blotting, Northern
Cell Communication physiology
Cells, Cultured
Interleukin-1 genetics
Interleukin-1 metabolism
Male
RNA, Messenger genetics
Rats
Rats, Sprague-Dawley
Sertoli Cells chemistry
Sertoli Cells metabolism
Sertoli Cells physiology
Spermatids metabolism
Spermatids physiology
Spermatocytes metabolism
Spermatocytes physiology
Testis cytology
Testis metabolism
Interleukin-1 analysis
RNA, Messenger analysis
Spermatids chemistry
Spermatocytes chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0303-7207
- Volume :
- 105
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecular and cellular endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 7859917
- Full Text :
- https://doi.org/10.1016/0303-7207(94)90177-5