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The mature form of interleukin-1 alpha is constitutively expressed in immature male germ cells from rat.

Authors :
Haugen TB
Landmark BF
Josefsen GM
Hansson V
Högset A
Source :
Molecular and cellular endocrinology [Mol Cell Endocrinol] 1994 Nov; Vol. 105 (2), pp. R19-23.
Publication Year :
1994

Abstract

In the present study we show that immature germ cells from rat testis contain both interleukin-1 alpha (IL-1 alpha) mRNA and immunoreactive proteins. In contrast, in primary cultures of Sertoli cells and peritubular cells. IL-1 alpha mRNA and immunoreactive protein were below the levels of detection. Immunoblots of lysates from these germ cells showed the presence of strong 17 kDa bands (mature forms of IL-1 alpha) and a much weaker 33 kDa band (precursor form). The finding of cell-associated mature forms of IL-1 alpha in germ cells indicates that immature male germ cells are able to process IL-1 alpha independent of its secretion. Data from isolated cell fractions, as well as from whole testis tissue from rats of various ages, indicate that IL-1 alpha expression takes place in late pachytene spermatocytes and early round spermatids. Whether IL-1 alpha plays a role intracellularly in germ cells or exerts its effects on neighboring Sertoli cells remains to be shown.

Details

Language :
English
ISSN :
0303-7207
Volume :
105
Issue :
2
Database :
MEDLINE
Journal :
Molecular and cellular endocrinology
Publication Type :
Academic Journal
Accession number :
7859917
Full Text :
https://doi.org/10.1016/0303-7207(94)90177-5