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Source of peritoneal proteoglycans. Human peritoneal mesothelial cells synthesize and secrete mainly small dermatan sulfate proteoglycans.
- Source :
-
The American journal of pathology [Am J Pathol] 1995 Feb; Vol. 146 (2), pp. 520-9. - Publication Year :
- 1995
-
Abstract
- This study describes experiments that compare the proteoglycans (PGs) extracted from the dialysate from patients receiving continuous peritoneal ambulatory dialysis (CAPD) with those secreted by metabolically labeled human peritoneal mesothelial cells in vitro. The PGs isolated from both sources were predominantly small chondroitin sulfate/dermatan sulfate PGs. Western blot of the core proteins obtained after chondroitin ABC lyase treatment with specific antibodies identified decorin and biglycan. With [35S]sulfate and [35S]methionine as labeling precursors it was shown that dermatan sulfate rather than chondroitin sulfate were the major glycosaminoglycan chains and that decorin was the predominant species. These data provide the first evidence that human peritoneal mesothelial cells may be the principal source of PGs in the peritoneum. Given the proposed functions of decorin and biglycan, the results suggest that these PGs may be involved in the control of transforming growth factor-beta activity and collagen fibril formation in the peritoneum.
- Subjects :
- Biglycan
Chondroitin Sulfates chemistry
Chondroitin Sulfates isolation & purification
Chondroitin Sulfates metabolism
Decorin
Dermatan Sulfate isolation & purification
Dermatan Sulfate metabolism
Epithelium metabolism
Extracellular Matrix Proteins
Humans
Proteoglycans chemistry
Ascitic Fluid chemistry
Kidney Failure, Chronic therapy
Peritoneal Dialysis, Continuous Ambulatory
Peritoneum metabolism
Proteoglycans isolation & purification
Proteoglycans metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9440
- Volume :
- 146
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The American journal of pathology
- Publication Type :
- Academic Journal
- Accession number :
- 7856761