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Source of peritoneal proteoglycans. Human peritoneal mesothelial cells synthesize and secrete mainly small dermatan sulfate proteoglycans.

Authors :
Yung S
Thomas GJ
Stylianou E
Williams JD
Coles GA
Davies M
Source :
The American journal of pathology [Am J Pathol] 1995 Feb; Vol. 146 (2), pp. 520-9.
Publication Year :
1995

Abstract

This study describes experiments that compare the proteoglycans (PGs) extracted from the dialysate from patients receiving continuous peritoneal ambulatory dialysis (CAPD) with those secreted by metabolically labeled human peritoneal mesothelial cells in vitro. The PGs isolated from both sources were predominantly small chondroitin sulfate/dermatan sulfate PGs. Western blot of the core proteins obtained after chondroitin ABC lyase treatment with specific antibodies identified decorin and biglycan. With [35S]sulfate and [35S]methionine as labeling precursors it was shown that dermatan sulfate rather than chondroitin sulfate were the major glycosaminoglycan chains and that decorin was the predominant species. These data provide the first evidence that human peritoneal mesothelial cells may be the principal source of PGs in the peritoneum. Given the proposed functions of decorin and biglycan, the results suggest that these PGs may be involved in the control of transforming growth factor-beta activity and collagen fibril formation in the peritoneum.

Details

Language :
English
ISSN :
0002-9440
Volume :
146
Issue :
2
Database :
MEDLINE
Journal :
The American journal of pathology
Publication Type :
Academic Journal
Accession number :
7856761