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Characterization of plasma membrane Na+/H+ exchange in eel (Anguilla anguilla) intestinal epithelial cells.
- Source :
-
The Journal of experimental zoology [J Exp Zool] 1995 Jan 01; Vol. 271 (1), pp. 18-26. - Publication Year :
- 1995
-
Abstract
- The ability of eel intestinal epithelial cells to recover from an acute acid load was analysed using the fluorescent dye 2',7'-bis-carboxy-ethyl-5,6-carboxyfluorescein (BCECF) and cell suspensions. Under these experimental conditions (bicarbonate-free solutions) the resting pHi in cells prepared from sea-water (7.52 +/- 0.031) and fresh-water (7.50 +/- 0.094) adapted animals proved to be similar. The recovery rate (following an acid load) increases by increasing the Na ion concentration in the extracellular medium. This pHi recovery is competitively inhibited by the specific inhibitor dimethylamiloride (DMA) with a low Ki in sea- (1.2 microM) as well as in fresh-water (1.3 microM) adapted animals, indicating the presence of a specific Na/H exchange activity in these cells. Using basolateral membrane vesicles it could be demonstrated that this activity is located on the basolateral side of the enterocyte membrane. The kinetic parameters (Kapp and Jmax) of this exchanger are similar in fresh-water and sea-water adapted animals suggesting that no salinity adaptation occurs, thus excluding the involvement of the antiporter in the osmoregulatory processes. These results are in agreement with the presence in the plasma membrane of the eel enterocytes of a Na/H-1 (housekeeper) form of the antiporter.
- Subjects :
- Amiloride analogs & derivatives
Amiloride metabolism
Animals
Buffers
Cell Membrane metabolism
Cell Survival
Epithelial Cells
Epithelium metabolism
Hydrogen-Ion Concentration
Intestinal Mucosa cytology
Potassium metabolism
Sodium metabolism
Eels metabolism
Intestinal Mucosa metabolism
Sodium-Hydrogen Exchangers metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-104X
- Volume :
- 271
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of experimental zoology
- Publication Type :
- Academic Journal
- Accession number :
- 7852945
- Full Text :
- https://doi.org/10.1002/jez.1402710103