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Overexpression of IL-10 in atopic dermatitis. Contrasting cytokine patterns with delayed-type hypersensitivity reactions.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 1995 Feb 15; Vol. 154 (4), pp. 1956-63. - Publication Year :
- 1995
-
Abstract
- The skin lesions of patients with atopic dermatitis provide a model to study immunoregulation in human allergy. To determine the local cytokine pattern of cells present (both endogenous and recruited) at the site of disease, we extracted RNA from skin biopsy specimens from patients with atopic dermatitis, allergic contract dermatitis, and positive tuberculin reactions and used PCR to assay for cytokine mRNA. cDNAs were normalized to the intensity of the CD3 delta PCR product as a marker of T cell mRNA. We found overexpression of IL-10 mRNA in atopic dermatitis lesions, in comparison with allergic contact dermatitis lesions and tuberculin reactions. In contrast, IL-4 mRNA was most strongly expressed in allergic contact dermatitis lesions and IFN-gamma mRNA was the predominant cytokine in tuberculin reactions. Using an anti-IL-10 mAb with immunoperoxidase, we localized IL-10 protein to large mononuclear cells in the dermal infiltrate of atopic lesions. After immunomagnetic sorting of mononuclear cell populations from PBMC of atopic dermatitis subjects, IL-10 mRNA as measured by PCR was found to be strongly expressed in CD14+ cells. Spontaneous release of IL-10 from PBMC-derived adherent cells was greater in atopic dermatitis donors than normal controls. We therefore renormalized skin biopsy cDNA according to the level of beta-actin PCR product, as a marker of total cellular mRNA, and found by PCR that IL-10 was nevertheless greatest in atopic dermatitis subjects. We conclude that the relative overexpression of IL-10 in atopic dermatitis greatest in atopic dermatitis subjects. We conclude that the relative overexpression of IL-10 in atopic dermatitis may contribute to the up-regulation of humoral responses and the down-regulation of Th1 responses.
- Subjects :
- Adult
Aged
Aged, 80 and over
Biopsy
DNA, Complementary genetics
Dermatitis, Atopic genetics
Dermatitis, Contact genetics
Dermatitis, Contact metabolism
Female
Gene Expression
Humans
Interferon-gamma biosynthesis
Interferon-gamma genetics
Interleukin-10 genetics
Interleukins biosynthesis
Interleukins genetics
Male
Middle Aged
Monocytes immunology
Monocytes metabolism
Polymerase Chain Reaction
RNA, Messenger analysis
Skin pathology
Skin Tests
Th2 Cells immunology
Th2 Cells metabolism
Tuberculosis, Pulmonary genetics
Tuberculosis, Pulmonary metabolism
Dermatitis, Atopic metabolism
Hypersensitivity, Delayed immunology
Interleukin-10 biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 154
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 7836775