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HLA class I self peptides isolated from a T-cell leukemia reveal the allele-specific motif of HLA-B38.
- Source :
-
Tissue antigens [Tissue Antigens] 1994 Aug; Vol. 44 (2), pp. 65-72. - Publication Year :
- 1994
-
Abstract
- Naturally-processed self peptides bound to HLA class I molecules of a T-cell leukemia (HLA-A1, A31, B38, B58) were isolated for sequence analysis. Acid-eluted peptides were subjected to reversed-phase HPLC separation and single-fraction sequencing was performed by Edman degradation. The peptides were found to be mostly nonamers and could be grouped into three distinct structural motifs. One of the peptide groups consistently displayed histidine at position 2 and a bulky hydrophobic residue at the C-terminus (XHXPXXXXY/F). The only HLA class I structure expressed by this T-cell leukemia which is consistent with the binding of peptides carrying this sequence motif is HLA-B38. A peptide binding assay confirmed this assignment. HLA-B38 is present in 10-12% of the Jewish population and is associated with several autoimmune disorders. The HLA-B38 motif may be an important tool for identifying potential T-cell epitopes involved in these diseases and for designing peptide vaccines.
- Subjects :
- Amino Acid Sequence
Antigen Presentation
Autoantigens immunology
Autoantigens isolation & purification
Autoantigens metabolism
Chromatography, High Pressure Liquid
Consensus Sequence
Gene Frequency
HLA-A1 Antigen immunology
HLA-B Antigens immunology
HLA-B Antigens isolation & purification
HLA-B38 Antigen
Humans
Jews genetics
Leukemia, Prolymphocytic, T-Cell immunology
Molecular Sequence Data
Neoplastic Stem Cells metabolism
Peptide Fragments chemistry
Protein Binding
Sequence Alignment
Sequence Homology, Amino Acid
T-Lymphocyte Subsets metabolism
Alleles
Autoantigens genetics
Genes, MHC Class I
HLA-A Antigens metabolism
HLA-A1 Antigen metabolism
HLA-B Antigens genetics
HLA-B Antigens metabolism
Leukemia, Prolymphocytic, T-Cell genetics
Peptide Fragments isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 0001-2815
- Volume :
- 44
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Tissue antigens
- Publication Type :
- Academic Journal
- Accession number :
- 7817380
- Full Text :
- https://doi.org/10.1111/j.1399-0039.1994.tb02361.x