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Expression of all-trans-retinoic acid receptor RNA in human thyroid cells.
Expression of all-trans-retinoic acid receptor RNA in human thyroid cells.
- Source :
-
Biochemistry and molecular biology international [Biochem Mol Biol Int] 1994 Aug; Vol. 33 (6), pp. 1107-15. - Publication Year :
- 1994
-
Abstract
- The expression of all-trans-retinoic acid receptor (RAR) RNA was investigated by Northern blot and Reverse Transcription-Polymerase Chain Reaction in tissues and primary cultures of human thyrocytes. In normal and adenomatous samples the RAR alpha RNA was expressed, whereas the expression of RAR beta and gamma was undetectable. In carcinoma samples RAR alpha RNA expression could decline, whereas the RAR beta RNA expression could become detectable. TSH and retinoic acid did not significantly modify RAR alpha mRNA levels, whereas RA caused a significant decrease in basal and TSH-induced thyroid peroxidase (TPO) mRNA levels, and a decrease in DNA synthesis. These results demonstrate that RAR alpha gene is predominantly expressed in human thyrocytes, and suggest a molecular link between this gene and the negative regulation by RA of proliferation and function of follicular cells.
- Subjects :
- Cell Division drug effects
Cells, Cultured
DNA Replication drug effects
Enzyme Induction
Humans
Iodide Peroxidase biosynthesis
Polymerase Chain Reaction methods
RNA, Messenger analysis
RNA, Messenger biosynthesis
Reference Values
Thyroid Gland cytology
Thyroid Gland drug effects
Thyrotropin pharmacology
Tretinoin metabolism
Tretinoin pharmacology
Adenoma metabolism
Gene Expression drug effects
Receptors, Retinoic Acid biosynthesis
Thyroid Gland metabolism
Thyroid Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1039-9712
- Volume :
- 33
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Biochemistry and molecular biology international
- Publication Type :
- Academic Journal
- Accession number :
- 7804136