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In vitro translation of D2 dopamine receptors and their chimaeras: analysis by subtype-specific antibodies.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1994 Dec 15; Vol. 205 (2), pp. 1460-6. - Publication Year :
- 1994
-
Abstract
- We have developed a range of specific anti-peptide antibodies directed against the D2 and D3 dopamine receptors, and their alternatively spliced isoforms. To demonstrate the subtype- and isoform-specificities of these antibodies we have expressed the receptors in an in vitro translation system and have immunoprecipitated the expressed proteins. We have shown that the D2 and D3 receptors unexpectedly exhibit different migration properties on SDS-PAGE, in addition to displaying a significant difference between their predicted and apparent molecular weights. We have used D2/D3 chimaeric receptors to show that the differential migration between the subtypes can be attributed to the N-terminal half of the receptor and propose that the highly hydrophobic nature of the receptor proteins underlies their anomalous migration properties.
- Subjects :
- Amino Acid Sequence
Animals
Antibody Specificity
Cloning, Molecular
Electrophoresis, Polyacrylamide Gel
Mice
Molecular Sequence Data
Peptides chemical synthesis
Peptides immunology
Protein Structure, Secondary
Receptors, Dopamine biosynthesis
Receptors, Dopamine chemistry
Receptors, Dopamine immunology
Receptors, Dopamine D2 chemistry
Receptors, Dopamine D2 immunology
Receptors, Dopamine D3
Recombinant Fusion Proteins chemistry
Recombinant Fusion Proteins immunology
Protein Biosynthesis
Receptors, Dopamine D2 biosynthesis
Recombinant Fusion Proteins biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 205
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 7802682
- Full Text :
- https://doi.org/10.1006/bbrc.1994.2829