Back to Search
Start Over
Influence of exogenous interleukin-2 concentration on the isolation of human immunodeficiency virus.
- Source :
-
Microbiology and immunology [Microbiol Immunol] 1995; Vol. 39 (2), pp. 117-21. - Publication Year :
- 1995
-
Abstract
- A significant increase (P = 0.015) in the HIV isolation rate from plasma samples was achieved by use of 10 U/ml exogenous interleukin-2 compared to 20 U/ml. The sensitivity rose from 0% to 29% in patients negative for p24 core antigen (P = 0.031) and from 71% to 86% in patients positive for p24 core antigen in plasma (P > 0.05). Titration of infectious HIV revealed that 10 U/ml interleukin-2 is the optimal concentration to isolate low numbers of infectious particles of HIV.
- Subjects :
- Acquired Immunodeficiency Syndrome blood
Acquired Immunodeficiency Syndrome microbiology
Cells, Cultured
DNA, Viral analysis
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
HIV Core Protein p24 analysis
HIV Infections microbiology
HIV-1 genetics
HIV-1 growth & development
Humans
Leukocytes, Mononuclear drug effects
Microbiological Techniques
Polymerase Chain Reaction
Sensitivity and Specificity
Viremia microbiology
HIV Infections blood
HIV-1 isolation & purification
Interleukin-2 pharmacology
Leukocytes, Mononuclear microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0385-5600
- Volume :
- 39
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Microbiology and immunology
- Publication Type :
- Academic Journal
- Accession number :
- 7783684
- Full Text :
- https://doi.org/10.1111/j.1348-0421.1995.tb02177.x