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Nucleotide sequence and analysis of a gene (chiA) for a chitinase from Streptomyces lividans 66.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 1993 Oct; Vol. 57 (10), pp. 1691-8. - Publication Year :
- 1993
-
Abstract
- A chitinase gene (chiA) from Streptomyces lividans was characterized and its nucleotides sequenced. Although the deduced amino acid sequence of chitinase A1 did not show any similarity to those of other Streptomyces chitinases that has been sequenced, the C-terminal part, containing both a putative catalytic domain and type-III-like repeating units, showed a similarity (36%) to that of chitinase D from Bacillus circulans. A site of initiation of transcription was found approximately 51 bp upstream from the GTG initiation codon. The promoter region of the chiA gene was subcloned on a 178-bp fragment into the promoter-probe vector pIJ486, resulting in the chitin stimulated expression of the neomycin resistance gene. One of the deleted subclones, which contained a 114-bp sequence upstream from the translation start codon, retained both chitin stimulated production and glucose repression. Chitin stimulated production was lost in an other deleted mutant containing the 104-bp upstream sequence.
- Subjects :
- Amino Acid Sequence
Base Sequence
Blotting, Southern
Chitin metabolism
Chitinases chemistry
Chitinases metabolism
Cloning, Molecular
Culture Media
Molecular Sequence Data
Plasmids
Promoter Regions, Genetic
Restriction Mapping
Sequence Homology, Nucleic Acid
Streptomyces genetics
Transcription, Genetic
Chitinases genetics
Genes, Bacterial
Streptomyces enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0916-8451
- Volume :
- 57
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 7764265
- Full Text :
- https://doi.org/10.1271/bbb.57.1691